Use the syringe to remove a small sample from the bottle of drug to be tested, using the method shown in Fig 13.10. Open the red capped container of extraction buffer and introduce two to three drops of this sample to the buffer container. Screw back the red cap and shake the bottle (Fig 13.6a).
Using the Cassette
Break off the tip of the red cap of the extraction buffer container (Fig 13.6b). Place the cassette on a clean surface and apply three or four drops of the liquid onto the sample well of the cassette. Wait five minutes before reading the test.
Fig 13.6a
Fig 13.6b
Reading the Test (Fig 13.7)
Negative Result: Two lines appear. A red line in the control region (C), and a red or pink line in the test region (T).
Positive: One red line in the control region (C). NO line appears in the test region (T). The absence of a test line indicates a positive result for Nembutal.
Invalid: NO line appears inthe control region (C).