2008 PBL Cat_mech_r.qxd:PBL Catalog.mech 11/29/07 1:51 PM Page 43
The cells, viruses, and standards used in the assays we offer are shown in the table below. The sensitivity range of each assay is listed in units of
active interferon per milliliter. All of the standards used in our bioassays have been calibrated to the International Reference Standards such as
those outlined by the National Institutes of Health [Pestka, S. (1986) Methods in Enzymology, 119, 14-23] and the World Health Organization.
Bioassay Services Offered
*Sensitivity
Cytokine (U/ml) Cell Virus Standards
Human IFN-α 15-30 MDBK VSV Human IFN-α 2a (NIH reference: Gxa01-901-535)
Human IFN-α 2-5 A549 EMCV Human IFN-α 2a (NIH reference: Gxa01-901-535)
!
Rhesus/Cynomolgus 2-5 A549 EMCV Human IFN-α 2a (NIH reference: Gxa01-901-535)
N
E
W
IFN-α**
Human IFN-β 2-5 A549 EMCV Human IFN-β (NIH reference: Gxb02-901-535)
Human IFN-γ 2-5 A549 EMCV Human IFN-γ (NIH reference: Gxg01-902-535)
Human IFN-ω 15-30 MDBK VSV Human IFN-ω (NIBSC Code: 94/754)
4-10 A549 EMCV
Mouse IFN-α 15-30 L929 EMCV Mouse IFN-α (NIH reference: Ga02-901-511)
Mouse IFN-β 5-10 L929 EMCV Mouse IFN-β (NIH reference: Gb02-902-511)
Mouse IFN-γ 5-10 L929 EMCV Mouse IFN-γ (NIH reference: Gg02-901-533)
Rat IFN-α ** 25-50 L929 EMCV Units are determined with respect to the Mouse
IFN-α/β standard (NIH reference: Gu02-901-511)
Rat IFN-β** 5-10 L929 EMCV Units are determined with respect to the Mouse
IFN-α/β standard (NIH reference: Gu02-901-511)
Rat IFN-γ ** 5-10 L929 EMCV Units are determined with respect to the Mouse
IFN-γ standard (NIH reference: Gg02-901-533)
MDBK = Bovine Kidney * Activity varies with cell line and virus
L929 = Mouse Fibroblast **In these assays, one unit is defined by comparison to the
VSV = Vesicular Stomatitis Virus
International standards established by WHO/NIH. For samples
EMCV = Encephalomyocarditis Virus
where no established standard exists, the closest homologue is
A549 = Human Lung Epithelial
used (mouse for rat, etc.).
www.interferonsource.com Tel: +1 732-777-9123
I
Fax: +1 732-777-9141
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