2008 PBL Cat_mech_r.qxd:PBL Catalog.mech 11/29/07 1:50 PM Page 12
Interferon
PBL’s Universal Type I Interferon is a unique hybrid interferon cells
1
. Since many mammalian interferons are difficult and expensive
constructed from the recombinant human interferons alpha A and to obtain, Universal Type I Interferon is an invaluable substitute for
alpha D. It is also known as Human Interferon Alpha A/D (Bgl II), the these interferons. Antiviral activities in different mammalian cells vary.
notation “Bgl II” representing the restriction enzyme site at which the For example, Universal Type I Interferon displays similar activity in
hybrid is assembled. (Refer to PBL product #11200-1) mouse cells as in human cells (see figure below), but is twice as active
in cow cells and eight times as active in cat cells (data not shown).
This interferon can substitute for many mammalian type I interferons
(e.g., alpha, beta, kappa, omega, tau) and has been tested in human, 1. Rehberg, E., et al. (1982). J. Biol. Chem. 257: 11497
monkey, mouse, rat, cat, bovine, rabbit, guinea pig, pig and hamster
Universal Type I IFN
Specific GenBank
Activity* Accession
Protein Form Source (U/mg) Number Catalog # Size
Human IFN-αA/D (Bgl ll) Frozen with a carrier protein E. coli-expressed 1.2 x 10
8
n/a 11200-1 1 x 10
5
U
Human IFN-αA/D (Bgl ll) Frozen with a carrier protein E. coli-expressed 1.2 x 10
8
n/a 11200-2 1 x 10
6
U
U = International Units *Biological activity for recombinant proteins measured on bovine MDBK cells with Vesicular Stomatitis Virus
(VSV) may vary. Please refer to lot-specific insert.
Figure on right: Interaction of a type I IFN with the extracellular region of its
cognate receptor chains (IFN-αR1 and IFN-αR2c) results in the
TYPE I INTERFERON ACTIVATION CASCADE
phosphorylation of the receptor associated kinases, Tyk2 and Jak1,
promoting phosphorylation of STAT1 and STAT2 (see figure to the right). The
STAT1/STAT2 heterodimer complexes with p48 (IRF9) forming the ISGF3
transcription complex that translocates to the nucleus and activates the
IFN-α
expression of specific genes containing ISRE binding sequences within their IFN-αR1
IFN-αR2c
promoter. [Type I interferons include IFN-α, IFN-β, IFN-ω, IFN-κ, and IFN-τ]
Ligand
Binding
IFN-α
k
1
Tvk2
Ja
k
1
Ja
Tvk2
Stat2
1
1
Stat2
Stat
Stat
1
Stat Stat2
p48
transcription
ISRE
Figure above: Comparison of the Activity of Human IFN-α2A, Mouse IFN-αA,
and ‘Universal’ IFN, on human and mouse cells. Specific activity of the three
IFNs was determined in an A549/EMCV CPE assay (top panel) and a
L929/EMCV CPE assay (bottom panel). Both Universal and Human IFN-α2A are
potent on human cells while the Mouse IFN-αA has little activity on these
cells. Likewise, the Mouse IFN-αA and Universal IFN are potent on mouse cells
while the Human IFN-α2A has little activity.
PBLInterferonSource Toll Free: 1 877- PBL-8881
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