2008 PBL Cat_mech_r.qxd:PBL Catalog.mech 11/29/07 1:50 PM Page 29
iLite
TM
Human Interferon Alpha Cell-Based Assay Kit
The iLite
TM
Human IFN-α Cell-Based Assay Kit (PBL product #51100-1) is during which time, the amount of active Luciferase enzyme molecules
based upon a conventional gene-reporter assay format that has been is directly proportional to the number of IFN-α molecules that have
modified and adapted for the quantitative determination of biologically bound to the cell receptor. The luminescent substrate for the luciferase
active IFN-α levels. enzyme are added and the reaction product measured in a
A normal IFN-α responsive gene has been carefully selected and luminometer.
replaced with firefly luciferase with modifications to maximize reporter The level of luciferase enzyme activity observed in the assay can be
gene expression. correlated to International Units (IU) of the IFN-α by the generation of a
The reporter cells used in the kit have been selected for a high level standard curve using the supplied IFN-α standard.
of active IFN-α receptor protein and are grown in a controlled manner
such that the number of receptors per cell is constant. They are then Features:
treated by a proprietary method to render them “cell proliferation • Novel gene-reporter based technology for the quantitative
deficient”. These division-arrested cells respond in a highly predictable determination of IFN-α biological activity
manner when used according to the assay instructions. • Detects multiple IFN-α subtypes
The iLite
TM
Human IFN-α Kit features a simple method for • Close correlation to anti-viral activity
quantitative determination of IFN-α levels. To quantify the amount of • Analyze Serum, Plasma, and Cell Culture Supernatant Samples fast
IFN-α in a biological sample, simply remove the kit from freezer, thaw with results in as little as 7 hours
the reporter cells and distribute to a 96-well plate. Then, just add • ISO 13485:2003 certified cell-based assay
samples and IFN-α standard. The cells are incubated for 7-17 hours,
Human IFN-α2a Dose Response Curve
Human IFN Dose Response Curve:
Alpha Subtype Specificity
120000
12.00
U) U)
100000
10.00
(RL (RL
t
s
t
s
8.00
ni
80000
ni
t
U
t
U
6.00
gh
60000
gh
Li Li
e 40000 e
4.00
a
t
iv
t
iv
2.00
R
e
l
20000
R
e
l
a
0 0.00
0.01 0.1 1 10 100 1000 0.10 1.00 10.00 100.00 1000.00 10000.00 100000.00
[IFN] (IU/ml) [IFN] (pg/ml)
iLite™Human IFN Alpha Cell-Based Assay
Matrix Sample
Type Compatibility Requirement Assay Range Catalog # Size
W!
Human IFN-α, Luminescent Cell Culture Supernatant, 25 µl 1.5-200 IU/ml 51100-1 1 Plate
NE
Cell-based Gene Reporter Assay Serum, and Plasma
1 Plate = 96 wells
www.interferonsource.com Tel: +1 732-777-9123
I
Fax: +1 732-777-9141
29
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