2008 PBL Cat_mech_r.qxd:PBL Catalog.mech 11/29/07 1:50 PM Page 28
VeriMax
TM
and VeriKine
TM
Signal Transduction ELISA Kits
Interferon signaling in the antiviral response is a complex and tightly- Signal transducer and activator of transcription (STAT) proteins are a
regulated cascade of events, as seen in the diagram on the inside seven-member family of cytoplasmic transcription factors that contribute
front cover. The players include ERKs, STATs, and a host of other to signal transduction by cytokines, hormones, and growth factors. STAT1
kinases and factors. promotes signaling of several cytokines including type I and type II
PBL InterefronSource is proud to introduce our new VeriMax
TM
and interferons through the JAK-STAT pathway. STAT3 is an intracellular protein
VeriPhos
TM
kits for the detection of IFN related signal transduction that exists in two distinct isoforms, full length STAT3α(86kDa) and STAT3β
products. These kits are based on the easy sandwich ELISA format. (79kDa). Many cytokines (IL-6, IFNs) and growth factors (p38 MAPK,
Accurate quantitative measurement can be made regardless of the ERK1 & 2) with vastly different biological functions have been shown to
level of phosphorylation of the target antigen. With the addition of these participate in the activation of STAT3 by promoting phosphorylation of the
signal transduction product to PBL’s existing products of IFN reagents protein at Tyr705 and Ser727, respectively.
and tools, scientists can now also study the downstream effects of The p38 MAPK (MAPK14) exists as four distinct isoforms in humans;
IFNs in their research. p38α, p38β/β2, p38γand p38δ. The p38αisoform has been shown to
The extracellular signal regulated kinases (ERKs) are be required for up-regulation of type I IFN (but not type II) gene
serine/threonine kinases that regulate gene expression in response expression. Consequently, p38α MAPK is required for the robust
to numerous extracellular signals including mitogens, antiviral properties of type I inteferons.
environmental stress, growth factors and cytokines. It remains Mouse TNF-α (Tumor Necrosis Factor-Alpha), also known as
unclear if interferons are involved in the up or down-regulation of cachectin, is a glycoprotein composed of 156 amino acids produced
ERK1/2 phosphorylation and activity. Nevertheless, combinations mainly by monocytes and activated macrophages. TNF-α has been
of ERK inhibitors and IFN have been shown to increase the shown to possess tumoricidal activity as well as a variety of
antiproliferative activity of IFN-α. physiologic effects with most major organ systems.
VeriMax™Signal Transduction Products ELISA
Matrix Sample
Type Compatibility Requirement Assay Range Catalog # Size
W!
ERK 1&2 (Total), Colorimetric Sandwich ELISA Cell Culture Supernatant 100 µl 0-2000 pg/ml 41800-1 1 Plate
NE
W!
Human STAT1 (Total), Colorimetric Sandwich ELISA Cell Culture Supernatant 100 µl 0-20 ng/ml 41810-1 1 Plate
NE
W!
Human STAT3 (Total), Colorimetric Sandwich ELISA Cell Culture Supernatant 100 µl 0-20 ng/ml 41820-1 1 Plate
NE
W!
p38 MAPK (Total), Colorimetric Sandwich ELISA Cell Culture Supernatant 100 µl 0-2000 pg/ml 41830-1 1 Plate
NE
1 Plate = 96 wells
VeriPhos™Signal Transduction Products ELISA
Matrix Sample
Type Compatibility Requirement Assay Range Catalog # Size
W!
NE
ERK 1&2 (pT185/pY187), Colorimetric Sandwich ELISA Cell Culture Supernatant 100 µl 0-100 U/ml 41805-1 1 Plate
W!
NE
Human STAT1 (pY701), Colorimetric Sandwich ELISA Cell Culture Supernatant 100 µl 0-100 U/ml 41815-1 1 Plate
W!
NE
Human STAT3 (pY705), Colorimetric Sandwich ELISA Cell Culture Supernatant 100 µl 0-100 U/ml 41825-1 1 Plate
W!
NE
p38 MAPK (pT180/pY182), Colorimetric Sandwich ELISA Cell Culture Supernatant 100 µl 0-100 U/ml 41835-1 1 Plate
1 Plate = 96 wells
PBLInterferonSource Toll Free: 1 877- PBL-8881
28
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