2008 PBL Cat_mech_r.qxd:PBL Catalog.mech 11/29/07 1:50 PM Page 21
Application Note
Table 1
% Spike Recoveries
Average % Spike
Matrix 25 pg/ml 250 pg/ml 500 pg/ml Recovery from matrix
75% Human Plasma with Na-EDTA 95.76 103.9 89.45 96.35
50% Human Plasma with Na-EDTA 86.38 99.78 86.35 90.83
Sample Diluent 99.68 97.59 96.39 97.88
Table 2
Mean StDev Mean Mean Mean
Matrix OD Blanks Blanks OD
S7
LLOQ MSL OD
S1
% Accuracy
75% Human Plasma with Na-EDTA 0.077 0.008 1.983 0.117 0.093 0.161 95.66
50% Human Plasma with Na-EDTA 0.080 0.017 1.882 0.165 0.114 0.182 93.70
25% Human Plasma with Na-EDTA 0.099 0.012 2.291 0.159 0.123 0.191 97.47
Sample Diluent 0.074 0.004 2.543 0.094 0.082 0.200 98.14
Table 3
Mean StDev Mean Mean Mean
Matrix OD Blanks Blanks OD
S7
LLOQ MSL OD
S1
% Accuracy
75% Human Plasma with Na-Citrate 0.106 0.013 2.477 0.171 0.132 0.219 98.96
50% Human Plasma with Na-Citrate 0.121 0.007 2.421 0.156 0.135 0.198 97.95
25% Human Plasma with Na-Citrate 0.108 0.012 2.399 0.168 0.132 0.199 97.67
Sample Diluent 0.135 0.009 2.811 0.181 0.154 0.224 98.89
Table 4
Mean StDev Mean Mean Mean
Matrix OD Blanks Blanks OD
S7
LLOQ MSL OD
S1
% Accuracy
75% Human Plasma with Na-Heparin 0.089 0.006 2.109 0.119 0.101 0.178 98.80
50% Human Plasma with Na-Heparin 0.086 0.021 2.268 0.191 0.128 0.195 99.26
25% Human Plasma with Na-Heparin 0.096 0.004 2.428 0.116 0.104 0.203 96.84
Sample Diluent 0.087 0.007 2.731 0.120 0.100 0.238 96.42
Table 1:% Spike Recovery of Human IFN-α from 75%, 50% Human Plasma in Na-EDTA prepared using Sample Diluent.
Tables 2-4: The mean OD for blanks, S1 (lowest point on the standard curve 12.5 pg/ml), S7 (the highest point on the standard curve 500 pg/ml), LLOQ (mean OD
blanks + 5 X StDev (blanks)), MSL (mean OD blanks + 2 X StDev (blanks)), and Mean % Accuracy (100% difference between the actual concentration of standard in the
curve and the extrapolated concentration for the same point on the curve) were calculated for 25% Human Plasma, 50% Human Plasma, 75% Human Plasma and
Sample diluent curves for Human Plasma prepared using Na-EDTA (Table 2), Human Plasma prepared using Na-Citrate (Table 3), and Human Plasma prepared using
Na-Heparin (Table 4).
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