Hydrogen Peroxide
www.chemetrics.com | 1-800-356-3072
Methods
Visual Kits
Hydrogen peroxide is a strong oxidizing agent with
a variety of uses. Applications include the treating of
industrial effluents and domestic waste and serving as
Range: 0-0.50 ppm
a disinfectant in aseptic packaging. MDL: 0.025 ppm / Method: DDPD
For the food and beverage industry, CHEMetrics
Cat#
Hydrogen Peroxide CHEMets
®
and Vacu-vials
®
products CHEMets Kit K-5504
are used extensively to monitor sterilization solutions in CHEMets Refill, 30 ampoules R-5504
the packaging and sanitizing processes.
Activator Solution Pack, six 10 mL bottles, Shelf-life 2 years A-2500
1
Activator Solution Pack, six 10 mL bottles, Shelf-life 2 years A-5500
1
The Ferric Thiocyanate Method
Comparator, Shelf-life 2 years:
0, 0.05, 0.10, 0.15, 0.20, 0.25, 0.30, 0.50 ppm C-5504
Reference: D. F. Boltz and J. A. Howell, eds.,
Kit comes in a plastic case and contains everything needed to perform 30 tests:
Colorimetric Determination of Nonmetals, 2
nd.
ed.,
Refill, Comparator, Activator Solutions, 25 mL sample cup, instructions, and
Vol. 8, p. 304 (1978).
MSDS.
The ferric thiocyanate method consists of ammonium
thiocyanate and ferrous iron in acid solution.
Range: 0-1 & 1-10 ppm
MDL: 0.05 ppm /Method: Ferric Thiocyanate
Hydrogen peroxide oxidizes ferrous iron to the ferric
Cat#
state, resulting in the formation of a red thiocyanate
CHEMets Kit K-5510
complex. Chlorine will not interfere with this method.
CHEMets Refill, 30 ampoules R-5510
Ferric iron will interfere. Results are expressed as ppm
Low Range Comparator, Shelf-life 2 years:
(mg/L) H
2
O
2
. 0, 0.1, 0.2, 0.3, 0.4, 0.6, 0.8, 1.0 ppm C-5501
High Range Comparator, Shelf-life 2 years:
The DDPD Method
1, 2, 3, 4, 5, 6, 7, 8, 10 ppm C-5510
Kit comes in a plastic case and contains everything needed to perform 30 tests:
Reference: Developed by CHEMetrics, Inc. Refill, Low and High Range Comparators, 25 mL sample cup, instructions, and
MSDS.
With the DDPD Method, hydrogen peroxide reacts
with DDPD in the presence of ammonium molybdate
1
The accessory pack supplies enough solution to perform at least
to form a purple product. Results are expressed as
200 tests.
ppm (mg/L) H
2
O
2
.
The Ceric Sulfate Titrimetric Method
Reference: Developed by CHEMetrics, Inc.
CHEMetrics developed a titrimetric method using ceric
sulfate as the titrant and ferroin as the end point indi-
cator. A color change from green to orange signals the
end of the titration. Results are expressed as percent
(%) H
2
O
2
.
The Ceric Sulfate Go-No-Go Method
Reference: Developed by CHEMetrics, Inc.
Developed for clinical applications where hydrogen
peroxide in sterilizing/disinfecting solutions with a
MEC (minimum effective concentration) of 6.0 ± 1.0%
must be monitored for efficacy. A single, small dose of
sample is added to a screw cap vial containing ceric
sulfate and the endpoint indicator ferroin.
An immediate color change occurs to signal that the
hydrogen peroxide level in the sample is either above
or below 6.0%.
46
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