Ammonia
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Methods
Low-level ammonia nitrogen may be naturally present
The Salicylate Method offers similar sensitivity to the
in water as a result of the biological decay of plant and
Nesslerization Method and is available in both visual
animal matter. Higher concentrations in surface waters
and instrumental versions. The benefits that the
can indicate contamination from waste treatment facili-
Salicylate product line offers are that the shelf-life of
ties, raw sewage, industrial effluents (particularly from
the ampoules is unlimited and there is no generation of
petroleum refineries), or fertilizer runoff. Excessive
mercury-containing waste.
ammonia concentrations are toxic to aquatic life.
The Direct Nesslerization Method
Visual Kits
Reference: ASTM D 1426-03, Ammonia Nitrogen in
Range: 0-1 & 1-10 ppm
Water, Test Method A. APHA Standard Methods, 18
th
MDL: 0.05 ppm / Method: Direct Nesslerization
ed., pp. 4-78, Method 4500-NH
3
C (1992).
Cat#
The test kits employing the well-established Nessler
CHEMets Kit *K-1510
reagent* to determine ammonia concentrations are
CHEMets Refill, 30 ampoules, Shelf-life 5 months *R-1501
2
applicable to drinking water, clean surface water, good-
Stabilizer Solution Pack, six 10 mL bottles, Shelf-life 2 years A-1500
1
quality nitrified wastewater effluent, and seawater. Stabilizer Solution Pack, six 70 mL bottles, Shelf-life 2 years A-1501
1
In some waters, calcium and magnesium concentrations Low Range Comparator, Shelf-life 2 years:
can cause cloudiness of the reagent. Adding a few
0, 0.1, 0.2, 0.3, 0.4, 0.6, 0.8, 1.0 ppm C-1501
drops of stabilizer solution (Rochelle Salt) will prevent
High Range Comparator, Shelf-life 2 years:
1, 2, 3, 4, 5, 6, 7, 8, 10 ppm C-1510
this cloudiness. References recommend distilling
Kit comes in a plastic case and contains everything needed to perform
samples prior to analysis. Results are expressed as 30 tests: Refill, Low and High Range Comparators, Stabilizer Solutions,
ppm (mg/L) ammonia-nitrogen, NH
25 mL sample cup, 1.0 mL syringe, instructions, and MSDS.
3
-N.
Shelf-life: although the Nessler reagent is stable,
its high alkali content attacks the glass ampoule.
Range: 0-2 & 0-20 ppm
MDL: 0.05 ppm / Method: Salicylate
The resulting precipitate interferes with color compari-
Cat#
son. We recommend stocking quantities of CHEMets
®
CHEMets Kit K-1410
and VACUettes
®
ampoules that will be used within five
CHEMets Refill, 30 ampoules R-1401
months. A two-month supply of Vacu-vials ampoules is
Activator Solution Pack, six 20 mL bottles, Shelf-life 8 months A-1400
1
suggested. Refrigeration will dramatically extend the
shelf-life of these products.
Catalyzer Solution Pack, six 20 mL bottles, Shelf-life 2 years A-1401
1
*Contains mercury. Dispose according to local, state or federal laws.
Comparator, Shelf-life 2 years:
0, 0.25, 0.50, 0.75, 1.0, 1.25, 1.5, 1.75, 2.0 ppm C-1402
The Salicylate Method
Kit comes in a plastic case and contains everything needed to perform 30 tests
(except distilled water): Refill, Comparator, Activator Solution, Catalyzer
References: Krom, Michael D., Spectrophotometric
Solution, 25 mL sample cup, 3.0 mL syringe, instructions, and MSDS.
Determination of Ammonia: A Study of a Modified
Berthelot Reduction Using Salicylate and
Range: 0-30 & 30-300 ppm
Dichloroisocyanurate, The Analyst, V105, pp. 305-316,
MDL: 5 ppm / Method: Direct Nesslerization
1980.
Cat#
In the ammonia test method that employs the Salicylate VACUettes Kit *K-1510D
chemistry, free ammonia reacts with hypochlorite to VACUettes Refill, 30 ampoules, Shelf-life 5 months *R-1501D
2
form monochloramine. Monochloramine reacts with
Low Range Comparator, Shelf-life 2 years:
salicylate, in the presence of sodium nitro-ferricyanide,
0, 5, 7.5, 10, 15, 20, 25, 30 ppm C-1501D
to form 5-aminosalicylate, a green-colored complex.
High Range Comparator, Shelf-life 2 years:
30, 60, 90, 120, 150, 175, 200, 250, 300 ppm C-1510D
This test method measures free ammonia and mono-
Kit comes in a plastic case and contains everything needed to perform
chloramine. Results are expressed in ppm (mg/L) ammo-
30 tests (except distilled water): Refill, Low and High Range Comparators,
nia-nitrogen, NH
dilutor snapper cup, micro test tube, instructions, and MSDS.
3
-N.
*Contains mercury. Dispose according to local, state or federal laws.
18
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