Gene Transfer
Another Successful Block
Introducing siLentFect
™
lipid reagent for RNAi, an effective method for siRNA delivery —
from Bio-Rad, the leader in gene transfer technology.
1,000 100
O
Unsurpassed efficiency for exceptional
siRNA delivery
90
RFU
80
O
Excellent gene-specific knockdown
70
with as little as 5 nM siRNA
60
O
Low lipid volume requirement
subtracted 100
knockdownknockdown
50
line 40
O
Low toxicity at optimal delivery volumes
base
GAPDH GAPDH 30
% %
O
Simple protocol 20
PCR
10
For more information, data, and
0 0
promotional offers, visit us on the Web 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 44 46 48 50
at www.bio-rad.com/ad/siLentFect/
Cycle
GAPDH knockdown >95% in HeLa cells using siLentFect and pooled siRNA. HeLa cells
were transfected in 6-well plates with 2.5 µl siLentFect using 10 nM GAPDH SMARTpool
siRNA ( ) or a nonspecific control pool ( ). Cells were harvested 48 hr posttransfection and
RNA extracted using the Aurum
™
total RNA kit. cDNA was produced using iScript
™
cDNA
synthesis kit and quantitative PCR performed using the iCycler iQ
®
system.
SMARTpool is a trademark of Dharmacon, Inc.
To fi nd your local sales offi ce, visit www.bio-rad.com/contact/
In the U.S., call toll free at 1-800-4BIORAD (1-800-424-6723)
Visit us on the Web at discover.bio-rad.com
BioRad_GeneTransfer.indd 1 07-0759 siLentFect_soc_ad.indd 10/12/07 4:15:01 PM
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