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| Multitask Microscopy
Live Cell Imaging
Olympus Life Science Europa GmbH
Wendenstrasse 14-18
D-20097 Hamburg
Germany
Phone: +49 40 23773-0
microscopy@olympus-europa.com
www.olympus-europa.com
From All to Small and Back
Imaging the Whole Organism
screen out unsuitable specimens (no signal) without blocking important
In some respects scientifi c research has come full circle: Scientists
and expensive resources such as cLSMs. As a result, research becomes
started looking at whole organisms and then drilled down to the molecular
more time and cost effi cient and is also accelerated.
level. Now they are beginning to look at the whole organism again, but
with a better knowledge of the fundamental molecular building blocks. Highly Sensitive and Fast Imaging
This new ‘physiology’ research has led Olympus to develop a number of of Small Laboratory Animals
new instruments such as the SZX2 stereo microscope series, the MVX10 The OV110 imaging system uses a combination of motorized
Macroscope and OV110 whole animal imaging system. The sensitivity magnifi cation changer, high NA optics, heated chamber (plus optional
and resolution of these microscope systems drive 3D fl uorescence anaesthesia equipment), an advanced zoom system, and digital
work to new levels, enabling researchers to gain new insights into the camera to provide whole animal to cellular level bio-illuminated (e.g.,
complex mechanisms of life. fl uorescence, etc.) imaging in live samples. As a result, the OV110
delivers the optimum clarity at all levels: anatomical, tissue, vascular
The Ultimate 3D View and cellular. These features combined with the latest fl uorescent dyes
With our visual system tuned to seeing things in three dimensions, and advanced post-acquisition processing software make the OV110
fl at images, even in high resolution can fail to successfully show the perfect for a large number of investigations, such as the extravasation
information fully. At higher magnifi cations though, there is not enough of labeled cancer cells, the growth/shrinking of tumors, bone healing as
depth of fi eld to generate a 3-dimensional view. Therefore, stereo well as the blood system, for example.
microscopy has always had a place in the low magnifi cation observation
of samples that have enough depth information. The SZX2 range from
Image 1: 28-30 hour old zebrafish
(Danio rerio) embryos expressing
Olympus has taken stereo microscopy a step further, providing high
green fluorescent protein (GFP).
resolution and high fi delity images across a very broad magnifi cation
range. The UIS2 optics also enable an unprecedented fl uorescence
capability through the use of high numerical apertures (NAs) and
autofl uorescence-free glass. This maximizes the light available for each
of the two optical paths in the Galileo-based arrangement. This plus
other features enable the SZX2 series to offer the best all round low
Image 2: A young Medaka one day
after hatching with macrophages
magnifi cation imaging systems.
labeled with membrane YFP driven by
a macrophage specific promoter.ak
High Sensitivity Fluorescence Zoom Macroscope on an MVX10. Courtesy of Dr. Adam
The MVX10 provides the ultimate in fl uorescence microscopy at low Cliffe, European Molecular Biology
magnifi cations with a specially developed optical system using very
Laboratory (EMBL), Heidelberg,
high NA objectives. As a result they provide excellent resolution and
Germany.
extremely effi cient light collection even with its large working distances
(WDs). This means that the faintest of fl uorescent signals from an entire
organism such as a C. elegans or a zebrafi sh (Dario rerio) can be viewed
at low magnifi cation and then zoomed in on for a closer look and further
investigation. This seamless process is possibly due to the parfocal
design of the objectives, the precision zoom optics and the two position
revolving nosepiece. These outstanding features mean that the MVX10
is an excellent tool for the pre-screening of samples, for example, which
improves workfl ows and effi ciency. Identifi cation of interesting regions
Image 3: Mouse showing bone
within an entire organism can be done quickly and easily. Since this is
remodelling in real time using
OsteSense
TM
. For NIRF imaging
not so easy with high magnifi cation systems such as confocal laser
the 0.14x and 0.8x objectives of
scanning microscopes (cLSMs), researchers can save a lot of time
OV110 have been used.
in screening whole organisms using an MVX10. This allows them to
Protocol Guide ı 2008 View entire protocol online at www.biotechniques.com/protocol ı BioTechniques ı 61
PGNov08-Olympus.indd 61 10/26/07 11:59:17 AM
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